Biological drug-expressing cell lines are constructed using Chinese Hamster Ovary (CHO) cells, which are used for most monoclonal antibodies and recombinant proteins on the market. Our company adopted DHFR-deficient CHO cells to take advantage of gene amplification capability in order to achieve a higher protein expression level. The CHO host cells are obtained from Dr. Chasin’s lab at Columbia University in the USA, with full license for commercial applications. 

      Our company has also established a cell line development platform using CHO-k cells. Depending on the project, the most suitable cell line development system will be chosen.

      The CHO host cells were adapted to a serum-free suspension culture in-house before being used for cell line development work.

      With our CHO host cells and proprietary expression vectors, our cell line development technical platform can routinely deliver high-quality cell lines suitable for manufacturing, with consistent protein production at a high expression level. Eight commercial cell lines for both monoclonal antibodies and recombinant proteins have been constructed using this system.

      The cell lines show an average yield of 2-3g/L, with data showing stability up to 60 generations as required for large-scale manufacturing. Protein samples are available for characterization six weeks after the initiation of cell line work, and further optimization and screening is carried out as needed. The quality of the cell line allows for consistency in the manufacturing process and ensures product quality. Our cell line team combines knowledge of process development with cell line creation, making the cell line suitable for use under manufacturing conditions. Our cell line has provided guaranteed performance over the course of the drug development process.